A comprehensive approach for mapping transgene integration sites & validating genome editing events
Recorded Thursday 28 April 2022
The ability to precisely manipulate the genome has driven major advances in biomedical research. Among the many editing tools at our disposal, CRISPR has emerged as a substantial one thanks to its ease-of-use, reduced cost and improved efficiency compared to ZFNs and TALENs. As remarkable as these technologies are, they each come with their own set of advantages and limitations. Notably, several studies have reported that repair of double-stranded breaks induced by CRISPR-Cas9 can set off significant on-target mutagenesis (e.g. large deletions and more complex genomic rearrangements at the targeted sites).
While several techniques exist for either random or targeted transgene integrations, the possibility for undesired (off-target) integrations, multiple integration sites, unexpected integration of backbone sequences should also not be overlooked. Similarly, undesired sequence or structural variants in the integrated transgene sequence and surrounding host genome sequence should not be neglected.
As a result, there is a clear need - within the biomedical community - for careful controls strategies to screen for such undesired outcomes. With it, we have been witnessing a surge in interest for robust assays capable of resolving complex rearrangement (in some instances, these can even extend over many kilobases).
Watch this webinar to discover how our proprietary TLA-based solutions have been gaining traction over the years and applied as the tool of choice for many researchers globally to identify all the genetic outcomes from their gene-editing events.
Expect to learn more about:
- Genetic QC challenges our customers typically face
- Published case studies demonstrating how to alleviate those genetic QC pains
- How our proprietary assays fare against conventional technologies (e.g. WGS, Southern Blot, etc.)
- Robust detection of all genetic variation, including structural variants, in and around genes of interest